Primary Clarification of CHO Harvested Cell Culture Fluid using an Acoustic Separator
نویسندگان
چکیده
منابع مشابه
Scale-up and optimization of an acoustic filter for 200 L/day perfusion of a CHO cell culture.
Acoustic cell retention devices have provided a practical alternative for up to 50 L/day perfusion cultures but further scale-up has been limited. A novel temperature-controlled and larger-scale acoustic separator was evaluated at up to 400 L/day for a 10(7) CHO cell/mL perfusion culture using a 100-L bioreactor that produced up to 34 g/day recombinant protein. The increased active volume of th...
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Automated monitoring of cell concentration in perfusion bioprocesses facilitates the maintenance of constant cell specific perfusion rates. However, most on-line measuring devices are relatively complex and foul as the culture progresses. A simple external optical sensor was developed using the transparent glass walls of acoustic separators for automated optical analysis of their contents. For ...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2020
ISSN: 1940-087X
DOI: 10.3791/61161